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Tuesday, January 30 • 11:00am - 11:20am
ASIAN CARP & OTHER AQUATIC INVASIVES: Detecting Round Goby in the Field Using a Portable eDNA Detection Kit

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AUTHORS. Christopher M. Merkes, Matthew Hoogland, Theresa Schreier, Jon J. Amberg - U.S. Geological Survey

ABSTRACT. Since Round Goby (Neogobius melanostomus) invaded the Great Lakes in the 1990s, they have been expanding their range inland. Monitoring this expansion can be difficult, because they are elusive to traditional survey gears. Environmental DNA (eDNA) analysis may be well suited to achieve early detection of Round Goby to guide more intensive surveys or rapid control action. We have developed a loop-mediated amplification (LAMP) assay that can be used with a field sample processing kit to detect Round Goby eDNA on site in under one hour. We did an evaluation of the assay at various sites within and ahead of the Round Goby invasion into the Fox River system near Appleton, Wisconsin. Alongside field processed samples analyzed with the LAMP assay, we also collected and analyzed traditional eDNA samples with qPCR for comparison.

Tuesday January 30, 2018 11:00am - 11:20am CST