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Tuesday, January 30 • 6:00pm - 9:00pm
Poster Display. Divergent Responsiveness of Two Isoforms of the Estrogen Receptor to Mixtures of Contaminants of Emerging Concern in Fathead Minnow and Bluegill Sunfish

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AUTHORS. Satomi Kohno, St. Cloud State University; Yoshinao Katsu, Hokkaido University; Nicholas Cipoletti*, Lina C. Wang, Zachary G. Jorgenson – St. Cloud State University; Shinichi Miyagawa, Wakayama Medical University; Heiko L. Schoenfuss, St. Cloud State University

ABSTRACT. Contaminants of emerging concern (CECs) are ubiquitous in aquatic environments with well-established endocrine-disrupting effects.  A data matrix of 559 water samples was queried to identify two commonly occurring CECs mixtures in Great Lakes tributaries.  One mixture consisted of eight agricultural CECs (AG), while another contained eleven urban CECs (UB).  The known estrogenic compounds bisphenol-A, estrone and nonylphenol were present in both mixtures.  According to the EPA ToxCast database, AG and UB mixture at an environmentally relevant concentration were estimated to account for 6.5% and 3.4% estrogenicity of the model endocrine disruptor estradiol-17ß (E2), respectively.  Two isoforms of the estrogen receptor (Esr) cloned from fathead minnow, bluegill sunfish, American alligator and human, responded differently to AG and UB mixtures.  Human and bluegill Esr1 were the most sensitive to AG and UB mixtures, respectively.  Fathead minnow Esr1 and Esr2b were the least sensitive to 10x AG and UB in estrogen dose equivalents, respectively.  Even at environmentally documented concentrations, UB significantly activated bluegill Esr1.  Moreover, 100x concentrated AG or UB hyper-stimulated fathead minnow Esr1, American alligator Esr2 and human Esr2 beyond the maximum induction of E2.  These results indicate that efficacious receptors and species differ in their response to CECs mixtures.  Furthermore, estrogenicity may be present in some CECs not previously considered estrogenic, or, alternatively, estrogenicity of a mixture may be enhanced through chemical interactions.  Our study highlights the need for further studies of CECs utilizing a variety of receptors cloned from diverse species.

Tuesday January 30, 2018 6:00pm - 9:00pm CST
Ballroom C & Foyer

Attendees (5)