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Tuesday, January 30 • 6:00pm - 9:00pm
Poster Display. Parental Reconstruction of Wild-Caught Young of Year Black Carp

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AUTHORS: Zebadiah Woiak, Emy M. Monroe, Erica L. Mize, U.S. Fish & Wildlife Service

ABSTRACT: In the fall of 2016, presumed young of year (YOY) Black Carp Mylopharyngodon piceus were discovered in a small agricultural ditch approximately 8 miles upstream of the ditch confluence with a navigable diversion channel and the Mississippi River near Cape Girardeau, Missouri. Missouri Department of Conservation (MDC) and U.S. Fish and Wildlife Service staff collected 64 presumed YOY Black Carp during multiple sampling events, and were genetically identified to confirm species. Sixty three samples were identified as Black Carp using the cytochrome c oxidase 1 (COI) and cytochrome b (cyt b) mitochondrial genes. Confirmed Black Carp were genotyped at 18 microsatellite loci, and analyzed as one population for population-level analysis. Genetic diversity (mean observed heterozygosity (Ho), mean allelic richness (Ar) and effective population size (Ne)) were calculated and parental reconstruction was performed using two different software programs. Mean Ho and Ar were 0.66 (SD=0.01) and 5.78 (SD=2.65), respectively. Effective population size was 25 (95% CI=15-44). Both parental reconstruction programs estimated 36 parents (18 males and 18 females) contributed to the progeny. Full sibling groups ranged from 11 to 1 offspring. Back-calculated Julian hatch dates provided by MDC corroborate our results for full sibling groups, thus we are confident the YOY Black Carp came from multiple parental spawning events. Our results coupled with MDC hatch date data provides valuable information about the population front of adult Black Carp that will be used to guide future early detection and monitoring efforts for Black Carp in the Mississippi River.

Tuesday January 30, 2018 6:00pm - 9:00pm CST
Ballroom C & Foyer

Attendees (2)